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ASCP ASCP-MLT Exam Syllabus Topics:
| Section | Weight | Objectives |
|---|---|---|
| Topic 1: Microbiology | 15-20% | - Identification of pathogenic microorganisms - Specimen collection and culture techniques - Bacteriology and antimicrobial susceptibility - Mycology, parasitology, and virology |
| Topic 2: Clinical Chemistry | 20-25% | - Endocrinology and therapeutic drug monitoring - Carbohydrates, lipids, proteins, and enzymes - Electrolytes, acid-base balance, and blood gases - Toxicology and liver/kidney function tests |
| Topic 3: Blood Banking (Immunohematology) | 15-20% | - Compatibility testing - Antibody detection and identification - Blood components and storage - Blood group systems and antigens - Transfusion reactions and adverse events |
| Topic 4: Immunology and Serology | 5-10% | - Autoimmune and immunodeficiency disorders - Infectious disease serology - Immune system principles and components - Immunologic testing methodologies |
| Topic 5: Laboratory Operations | 5-10% | - Quality control and quality assurance - Laboratory safety and regulatory compliance - Laboratory mathematics and instrumentation - Pre-analytical, analytical, and post-analytical processes |
| Topic 6: Urinalysis and Other Body Fluids | 5-10% | - Cerebrospinal, serous, and synovial fluids - Physical, chemical, and microscopic analysis - Semen and amniotic fluid testing - Urine formation and composition |
| Topic 7: Hematology | 20-25% | - Hemostasis and coagulation testing - White blood cell morphology and diseases - Routine and automated hematology procedures - Red blood cell physiology and disorders |
ASCP MEDICAL LABORATORY TECHNICIAN - MLT(ASCP) Sample Questions:
Question 1
Malbranchia species share the production of alternate staining arthroconidia as a common feature with the mold form of Coccidioides immitis.
Both Geotrichum species and Trichosporon species produce rectangular-shaped arthroconidia; however, they are regularly rather than alternately staining. Additionally, the arthroconidia of Geotrichum may produce germ tubes from one corner and the arthroconidia of Trichosporon species may produce blastoconidia from adjacent corners, features not shared by either Malbranchia species or Coccidioides immitis.
The hyphae of Microsporum canis, as seen in direct KOH mounts of skin scales, may break up into arthroconidia; however, they are much narrower in dimension and do not share the alternate staining characteristics.
Microbiology
The hyaline saprobic fungus that has microscopic features similar to the mold form of Coccidioides immitis is:
A. Malbranchia species
B. Microsporum canis
C. Trichosporon species
D. Geotrichum species
Question 2
Platelets do not circulate in inactivated, spiny forms. The spiny, sticky form of the platelet is initiated once the platelets become activated in response to blood vessel damage.
Which of the following is not true in terms of platelet characteristics?
A. Are normally found circulating in their inactive, spiny form.
B. Possess an inherent sticky property that aids in adhesion and aggregation.
C. Have a lifespan of roughly 9-12 days.
D. Produced in the bone marrow by megakaryocytes.
Question 3
1. B
2. D
3. A
4. C
Red to Brown Urine: porphobilinogen, hematuria, myoglobinuria, etc.
Green: Food colorings; Increased carotene in the diet;
Pseudomonas aeruginosa infection
Yellow: bilirubin, bile pigments
White: phosphates, other crytals
Match urine color with substance that might have been responsible:
1. Phosphates
2. Bilirubin
3. Pseudomonas
4. Porphobilinogen
A. White
B. Blue to green
C. Yellow
D. Red to brown
Question 4
A laboratory scientist is working the night shift at a local hospital when the power goes out. What is the course of action to continue to provide laboratory results?
A. Wait until the physician calls asking for results
B. Wait until the power comes back
C. Hold all laboratory testing until the power comes back
D. Initiate the downtime protocol for an outage
Question 5
Basophilic stippling is the term used to describe red blood cells that contain tiny particles of RNA within their cytoplasm. Basophilic stippling is associated with many conditions, but is strongly associated with lead poisoning.
Multiple small, dark blue particles scattered throughout the cytoplasm of erythrocytes is/are called:
A. Basophilic stippling
B. Howell-Jolly bodies
C. Pappenheimer bodies
D. Heinz bodies
Solutions:
| Question 1 Answer: A | Question 2 Answer: B | Question 3 Answer: A,B,C,D | Question 4 Answer: D | Question 5 Answer: C |






